Year: 2013 | Month: December | Volume 2 | Issue 2
Study of Antioxidants’ Enzymes Kinetics,Superoxide Dismutase, Glutathione Peroxidase and Catalase, in Neonatal Sepsis
Abstract: <div>Context: sepsis is associated with severe oxidative stress. During sepsis, there</div><div>are several potential sources of reactive oxygen species, including the respiratory burst associated with neutrophil activation. Aim: We try to monitor the kinetics of the scavengers’ antioxidant enzymes, erthrocyte superoxide dismutase (SOD), whole blood glutathione peroxidase (GPX) and serum catalase in the pathogenesis of neonatal sepsis and the effect of</div><div>antibiotics therapy on their levels. Study Design: This study was carried out on blood samples from children suffering from sepsis proved by positive blood culture in addition to 11 healthy children. Blood samples were subjected for determination of antioxidant enzyme activities including red blood cell superoxide dismutase (SOD), whole blood glutathione peroxidase (GPx) and serum catalase Results; There was highly significant decrease in erythrocyte superoxide dismutase (RBC SOD) (P<0.001) and whole blood glutathione peroxidase (GPx) (P=0.01) at admission and after 48 hours of start of antibiotics therapy (P<0.001, for each) in patients compared with controls. There was highly significant increase in serum catalase level in patients at admission (P<0.001) and after 48 hours of antibiotics therapy compared with controls. The commonest isolated organism from blood culture was Staphylococci species (66.7%) with Staphylococcus aureus (40.0%)</div><div>and Staphylococci epidermidis (26.7%). There was significant decrease of SOD (P1=.0001) and GPx (P1=.049) at start of sepsis associated with Staphylococcus aureus infection. After 48 hours from clinical response to antibiotics therapy, both SOD and GPx had significant decrease with all types of bacteria isolated. </div><div><b>Conclusion:</b> Our study suggests that children with sepsis are susceptible to high oxidative stress which may play a role in the pathogenesis of sepsis. The</div><div>administration of antibiotics therapy was not associated with the improvement of level of superoxide dismutase and glutathione peroxidase, so the utility of</div><div>supplementation of antioxidant enzymes in neonates with septicemia needs further evaluation.</div>
Print This Article Email This Article to Your Friend
